A Novel Fibrinolytic Enzyme Extracted from the Earthworm, Lumbricus rubellus.
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چکیده
منابع مشابه
Purification and characterization of six fibrinolytic serine-proteases from earthworm Lumbricus rubellus.
The six lumbrokinase fractions (F1 to F6) with fibrinolytic activities were purified from earthworm Lumbricus rubellus lysates using the procedures of autolysis, ammonium sulfate fractionation, and column chromatography. The proteolytic activities on the casein substrate of the six iso-enzymes ranged from 11.3 to 167.5 unit/mg with the rank activity orders of F2 > F1 > F5 > F6 > F3 > F4. The fi...
متن کاملSome features of intestinal absorption of intact fibrinolytic enzyme III-1 from Lumbricus rubellus.
In order to investigate whether earthworm fibrinolytic enzyme III-1 (EFE-III-1) isolated from Lumbricus rubellus is capable of transporting into blood through intestinal epithelium and keeping its biological function in circulation, we have raised an antibody against EFE-III-1. The immunological results showed that 10-15% of intact EFE-III-1 was absorbed by the intestinal epithelium with the in...
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Earthworms express, as most animals, metallothioneins (MTs)-small, cysteine-rich proteins that bind d(10) metal ions (Zn(II), Cd(II), or Cu(I)) in clusters. Three MT homologues are known for Lumbricus rubellus, the common red earthworm, one of which, wMT-2, is strongly induced by exposure of worms to cadmium. This study concerns composition, metal binding affinity and metal-dependent protein fo...
متن کاملCeramide glycanase from the earthworm, Lumbricus terrestris.
Ceramide glycanase (CGase) is an enzyme that cleaves the linkage between the sugar chain and the ceramide. To make this enzyme readily available, we have developed a simple method for preparing it from the earthworm, Lumbricus terrestris. The method involves Bio-Gel A-0.5m, octyl-Sepharose and p-aminophenylthiogalactoside-agarose column chromatography. By gel filtration, the molecular mass of e...
متن کاملCrystal structure of earthworm fibrinolytic enzyme component B: a novel, glycosylated two-chained trypsin.
The earthworm fibrinolytic enzyme (EFE), belonging to a group of serine proteases with strong fibrinolytic activity, has been used in a mixture as an oral drug for prevention and treatment of thrombosis in East Asia. The EFE component b (EFE-b) is one of seven EFE components from Eisenia fetida, and among them it has nearly the highest fibrinolytic activity. Here, we report its crystal structur...
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ژورنال
عنوان ژورنال: The Japanese Journal of Physiology
سال: 1991
ISSN: 0021-521X
DOI: 10.2170/jjphysiol.41.461